| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148522 |
| Chromosome: | chromosome 9 |
| Location: | 3074512 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387100 | CGL12,PTST1 | (1 of 5) PTHR10343 - 5'-AMP-ACTIVATED PROTEIN KINASE , BETA SUBUNIT; Protein targeting to starch 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGCGTGCAAGCGACTCCGCCAATCAGTCT |
| Internal bar code: | CCTGTACGGTAGCGCTTCACGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 743 |
| LEAP-Seq percent confirming: | 98.7676 |
| LEAP-Seq n confirming: | 1122 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTATCCGCTCAACATGG |
| Suggested primer 2: | CGGCCTTTTACTGACATGGT |