| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148523 |
| Chromosome: | chromosome 10 |
| Location: | 5936398 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g462200 | HDA17,SRTA1,SRTA | Sir2-like NADH dependent histone deacetylase; (1 of 1) K11416 - mono-ADP-ribosyltransferase sirtuin 6 (SIRT6, SIR2L6) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGGTTTTTCTTCGGCTTTAAACGGTCAG |
| Internal bar code: | TTGCGAAGGTCGACCAGCTCGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 614 |
| LEAP-Seq percent confirming: | 99.8609 |
| LEAP-Seq n confirming: | 718 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGTTGCTCCTCTTCAGC |
| Suggested primer 2: | GTACAGGCCTTGTAGCTGCC |