| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148525 |
| Chromosome: | chromosome_9 |
| Location: | 3667661 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g390578 | FAP11 | sense | 5'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ACAGCAATATTGTGATATTGTAACCGCGAT |
| Internal bar code: | GAGATGAGGATGACGAGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 409 |
| LEAP-Seq percent confirming: | 99.3789 |
| LEAP-Seq n confirming: | 160 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACTTGGCGTACTCAGGCTC |
| Suggested primer 2: | GACGGCTGTGAAGCAGTACA |