| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148528 |
| Chromosome: | chromosome 9 |
| Location: | 4663146 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g396735 | (1 of 1) PTHR18895:SF3 - METHYLTRANSFERASE-LIKE PROTEIN 20 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AACGCACAGCAGTCGTGCCTGTACGACATG |
| Internal bar code: | GACGGAGGGTCGCATTGAGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 925 |
| LEAP-Seq percent confirming: | 99.2339 |
| LEAP-Seq n confirming: | 2202 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTGAAATGGCGAGTGTATG |
| Suggested primer 2: | CGTCTTGACCACGAAGTCCT |