| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148541 |
| Chromosome: | chromosome 16 |
| Location: | 673396 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| intergenic |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTGGGCATTGACGGCAACCTGATTCCCGC |
| Internal bar code: | CAGTCCTGGGGGGGCCGTCGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 282 |
| LEAP-Seq percent confirming: | 99.5889 |
| LEAP-Seq n confirming: | 7267 |
| LEAP-Seq n nonconfirming: | 30 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTTCGCCGACTTGCTTAG |
| Suggested primer 2: | TATGGTCAGGACAGCAGCAG |