Insertion junction: LMJ.RY0402.148547_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.148547
Chromosome:chromosome 7
Location:2937369
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g332750 PSL2 Protein of unknown function; (1 of 19) IPR000225//IPR016024 - Armadillo // Armadillo-type fold intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCTTTCAACCACACGCCCACGCCTCCATG
Internal bar code:TGTGAGTAAGCGGTACTTGTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:550
LEAP-Seq percent confirming:99.9245
LEAP-Seq n confirming:1323
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGTCCAGCTGCATCTTT
Suggested primer 2:GATTGTGGCTCTGGGTGAGT