| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148584 |
| Chromosome: | chromosome 1 |
| Location: | 4461179 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g030350 | CGL41 | Predicted protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAAGCGCATGCGTGTGGCATGCGGCTGA |
| Internal bar code: | ACGGGGACGGGGGTTTTCCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 573 |
| LEAP-Seq percent confirming: | 99.6566 |
| LEAP-Seq n confirming: | 2612 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCACCAACAGAAATGACACG |
| Suggested primer 2: | CACAGCCTCTCCTGTCCTTC |