| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148595 |
| Chromosome: | chromosome_12 |
| Location: | 94741 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g483950 | MDY4,MDH4 | NAD-dependent malate dehydrogenase, mitochondrial | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GATTGTTTGGCAAACAGGCCACCTTGGGTG |
| Internal bar code: | TAAGCGCCCTAAAAGGAATGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 747 |
| LEAP-Seq percent confirming: | 90.6097 |
| LEAP-Seq n confirming: | 3329 |
| LEAP-Seq n nonconfirming: | 345 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGGGTTACGCACGTCATTT |
| Suggested primer 2: | CTATAAGCTGCTTGGGGCTG |