Insertion junction: LMJ.RY0402.148625_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.148625
Chromosome:chromosome 12
Location:3112006
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre12.g499950 CPL18 (1 of 2) PF07819 - PGAP1-like protein (PGAP1); possible serine esterase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGTGTGTAGTGAGGGGGCTGTGGAGGGAA
Internal bar code:GCACCCTCATCCTCGCACGGCA

Confirmation - LEAP-Seq

LEAP-Seq distance:615
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CAAACACATTGTCCCACGAG
Suggested primer 2:CTTCCATGCGCTGTGTAGAA