| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148626 |
| Chromosome: | chromosome 17 |
| Location: | 1197901 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g704950 | (1 of 2) PTHR13140//PTHR13140:SF410 - MYOSIN // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGTTTGCAGAATGCAGCAGGCGCAGCCCAG |
| Internal bar code: | AAGTCGTCGGATAAGGTGGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 687 |
| LEAP-Seq percent confirming: | 99.9361 |
| LEAP-Seq n confirming: | 1564 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACAGAAGCTGCGTGAATGTG |
| Suggested primer 2: | GGGAGTGGACATAGCCTTCA |