| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148658 |
| Chromosome: | chromosome 17 |
| Location: | 2518921 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g716350 | GT90F32,GT90-32 | GT90 family protein 32; (1 of 52) PF05686 - Glycosyl transferase family 90 (Glyco_transf_90) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCAATGGGTGGGCTCAAAGGCGGTGCGGA |
| Internal bar code: | CGATACGCAACCTTCGAGGTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 597 |
| LEAP-Seq percent confirming: | 90.128 |
| LEAP-Seq n confirming: | 986 |
| LEAP-Seq n nonconfirming: | 108 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCATACTTCTTGCCACGTT |
| Suggested primer 2: | TACAACGTGAGCCTACTGCG |