| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148659 |
| Chromosome: | chromosome 9 |
| Location: | 876412 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g401551 | BHD1 | 3-beta hydroxysteroid dehydrogenase/isomerase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTACAGGGGCTTGAAACTACTGAATGACA |
| Internal bar code: | GGTGATAAGGCATCGCATTTCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 930 |
| LEAP-Seq percent confirming: | 99.7494 |
| LEAP-Seq n confirming: | 1194 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACAGGGTCGTACCAATGCT |
| Suggested primer 2: | GTACATGCAAATGCAGTGGG |