| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148677 |
| Chromosome: | chromosome 2 |
| Location: | 3598684 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095137 | PFR1 | (1 of 1) 1.2.7.1 - Pyruvate synthase / Pyruvic-ferredoxin oxidoreductase; Pyruvate ferredoxin oxidoreductase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAGGCGGGTCACCTCCCCTTGCTTGCTTG |
| Internal bar code: | TCCGCTTCCATTTAGGTTAGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 190 |
| LEAP-Seq percent confirming: | 99.5407 |
| LEAP-Seq n confirming: | 3684 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGATAGGTCATGGAGGCGT |
| Suggested primer 2: | TCCAGCAGCCGCTTATTAGT |