| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148731 |
| Chromosome: | scaffold 35 |
| Location: | 5979 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre35.g759247 | SRR5 | Scavenger receptor cysteine rich (SRCR) protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGTGTCAGGTGTCAGGTGATAAAGATGG |
| Internal bar code: | CGAATCACGGATTACACTCGTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 261 |
| LEAP-Seq percent confirming: | 97.9592 |
| LEAP-Seq n confirming: | 96 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCTGATTTTTCATGTCGT |
| Suggested primer 2: | CCGTACCTGTGCTCCGTACT |