| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148754 |
| Chromosome: | chromosome 1 |
| Location: | 4746424 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g032800 | MRS2,MRS6 | Mg2+ transporter protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCAGAAGCAAAGCGTCCCGACGTTGATA |
| Internal bar code: | TCATGTCGCACGGGTCTGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 34 |
| LEAP-Seq percent confirming: | 99.3548 |
| LEAP-Seq n confirming: | 154 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCATCGTCGACACCACGTA |
| Suggested primer 2: | CATTTCTAGCTACAGGCCGC |