Insertion junction: LMJ.RY0402.148773_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.148773
Chromosome:chromosome 13
Location:2586858
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre13.g581050 GT90F46,GT90-46 (1 of 11) 2.4.2.26 - Protein xylosyltransferase / Uridine diphosphoxylose-protein xylosyltransferase; GT90 family protein 46 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATCGTCGCTCACGGCCTCCCGCCTCTCC
Internal bar code:ACACTGATTGTTGGTCGCGTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:916
LEAP-Seq percent confirming:97.3207
LEAP-Seq n confirming:2252
LEAP-Seq n nonconfirming:62
LEAP-Seq n unique pos:15

Suggested primers for confirmation by PCR

Suggested primer 1:CTGTTACAGACGGGGCATTT
Suggested primer 2:CAAGAAATCCAGCGGATCAT