| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148808 |
| Chromosome: | chromosome 2 |
| Location: | 3321223 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g095089 | (1 of 2) 3.6.1.19 - Nucleoside-triphosphate diphosphatase / Nucleoside-triphosphate pyrophosphatase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATACTGATAGTTTAGGTTAAGGCTAGGTT |
| Internal bar code: | CCGCATGATTGGCGTCCTCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 549 |
| LEAP-Seq percent confirming: | 99.8119 |
| LEAP-Seq n confirming: | 2122 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTGTGCACGCGATTTGTA |
| Suggested primer 2: | GGGGCTGCAAAAGAATGATA |