| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148847 |
| Chromosome: | chromosome 6 |
| Location: | 3841845 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278171 | CGL132 | predicted protein; (1 of 14) PF04720 - PDDEXK-like family of unknown function (PDDEXK_6) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTGCACATAGCTGACAAGAGACATCACA |
| Internal bar code: | CGCCAGGACGGCTCAGCGGCAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 106 |
| LEAP-Seq percent confirming: | 59.0214 |
| LEAP-Seq n confirming: | 579 |
| LEAP-Seq n nonconfirming: | 402 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCAGCTCCAGCTCGTTAG |
| Suggested primer 2: | TCGTCTACACAGCTCATCCG |