| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148855 |
| Chromosome: | chromosome_7 |
| Location: | 3786036 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre07.g338350 | CPLD50 | Predicted protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCCTTCTCATGGCCAACTTCGGGTTCTAGG |
| Internal bar code: | ATGCGCGCAGACTCGAGTTGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 138 |
| LEAP-Seq percent confirming: | 99.6942 |
| LEAP-Seq n confirming: | 326 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTGTCATACCGCTGCTGTC |
| Suggested primer 2: | GCAGATGTGACACAAGCGAT |