Insertion junction: LMJ.RY0402.148855_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.148855
Chromosome:chromosome 7
Location:3786036
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g338350 CPLD50 Conserved in the Plant Lineage and Diatoms; (1 of 1) PTHR10072//PTHR10072:SF43 - IRON-SULFUR CLUSTER ASSEMBLY PROTEIN // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCTTCTCATGGCCAACTTCGGGTTCTAGG
Internal bar code:ATGCGCGCAGACTCGAGTTGCG

Confirmation - LEAP-Seq

LEAP-Seq distance:138
LEAP-Seq percent confirming:99.6942
LEAP-Seq n confirming:326
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:TCTGTCATACCGCTGCTGTC
Suggested primer 2:GCAGATGTGACACAAGCGAT