| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148863 |
| Chromosome: | chromosome 14 |
| Location: | 422568 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g610663 | (1 of 1) PTHR12801//PTHR12801:SF51 - EXONUCLEASE // PROTEIN Y56A3A.33 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAAATCACATTCTGCGATCATCTAGAGT |
| Internal bar code: | GCGCTGACACAGTCGCCCGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 785 |
| LEAP-Seq percent confirming: | 99.7332 |
| LEAP-Seq n confirming: | 7477 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 33 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCACAAGGGCACCAACAG |
| Suggested primer 2: | TCAAGTCATGCCGCTATCAG |