| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148873 |
| Chromosome: | chromosome 7 |
| Location: | 5787682 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g353100 | (1 of 4) PTHR10229//PTHR10229:SF0 - GTP-BINDING PROTEIN HFLX // GTP-BINDING PROTEIN 6-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTGGATCCTGAACTCGTGGTGCTTATGG |
| Internal bar code: | ATGTTGGAATAAAGTACGTAAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 288 |
| LEAP-Seq percent confirming: | 99.7715 |
| LEAP-Seq n confirming: | 1310 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTTCCACCAAAGTGCAGGT |
| Suggested primer 2: | CTTCACTACCGCGCTACCAG |