| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.148917 |
| Chromosome: | chromosome 17 |
| Location: | 2715194 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g718100 | (1 of 1) PTHR23324:SF56 - CRAL-TRIO DOMAIN-CONTAINING PROTEIN YKL091C-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTTAAGGGCTCAGGGCTATAAATCTGTC |
| Internal bar code: | TGCATCACGCCAAGCTGGCGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 84.4677 |
| LEAP-Seq n confirming: | 7744 |
| LEAP-Seq n nonconfirming: | 1424 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCGAGTGTGCAGAGTAAC |
| Suggested primer 2: | GCCAGCATAGCTGTGTCGT |