| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148923 |
| Chromosome: | chromosome 3 |
| Location: | 2175010 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g157400 | (1 of 1) IPR000595//IPR014710//IPR018490 - Cyclic nucleotide-binding domain // RmlC-like jelly roll fold // Cyclic nucleotide-binding-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCACCGCCCACTCCCCACCCCGCCCTTTC |
| Internal bar code: | AGCTGGAGTAGCTATTGACTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 711 |
| LEAP-Seq percent confirming: | 98.5714 |
| LEAP-Seq n confirming: | 483 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TAGCCATCCGTTTACCAACC |
| Suggested primer 2: | AGTGAGGACAGGAAAGGGGT |