Insertion junction: LMJ.RY0402.148926_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.148926
Chromosome:chromosome 2
Location:2509023
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre02.g092200 QAD1 (1 of 8) IPR011047//IPR018391 - Quinonprotein alcohol dehydrogenase-like superfamily // Pyrrolo-quinoline quinone beta-propeller repeat; Quinonprotein alcohol dehydrogenase-like protein CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCTGCAGGGACGCACCTCAGAGGCCGCTCT
Internal bar code:ACGTGGAGTAAACGGCGCAGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:68.9873
LEAP-Seq n confirming:109
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CGGACTTCTTTAGCTCGGTG
Suggested primer 2:GGTCTCCGTTGTTGTCCAGT