| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148928 |
| Chromosome: | chromosome_3 |
| Location: | 4256927 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre03.g174250 | FAP111 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCTGCTGTGCCACCATCTGGCCATGGCGCC |
| Internal bar code: | TAGGGTGGGCTATCCGCAAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 185 |
| LEAP-Seq percent confirming: | 77.2727 |
| LEAP-Seq n confirming: | 34 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATCCTCAATGGTGGTGAGC |
| Suggested primer 2: | CCGCACTGCATCTTGAACTA |