| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.148941 |
| Chromosome: | chromosome 10 |
| Location: | 909746 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g424300 | KIN16A,KIN12-2,KIN16-1 | (1 of 32) 3.6.4.4 - Plus-end-directed kinesin ATPase / Kinesin; Kinesin motor protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTGATGGGCACGGGCCGCACAGCGCTTCG |
| Internal bar code: | ATGTTCCAGTCTGAGTGGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 290 |
| LEAP-Seq percent confirming: | 99.7974 |
| LEAP-Seq n confirming: | 3940 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCTAGAACTCACTGCCC |
| Suggested primer 2: | ACGGCAATGATTACTACGGC |