| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148971 |
| Chromosome: | chromosome 2 |
| Location: | 311180 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075350 | CNK1 | NimA-related protein kinase; (1 of 1) PTHR24361//PTHR24361:SF410//PTHR24362 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SUBFAMILY NOT NAMED // SERINE/THREONINE-PROTEIN KINASE NEK | 3'UTR|outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAACCAGTCGGGCGGCTCGGGCCGCATC |
| Internal bar code: | GGAGATCGGGCTCGGGAGTGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 377 |
| LEAP-Seq percent confirming: | 99.6223 |
| LEAP-Seq n confirming: | 1055 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTACGTTCTTGCTGGCTTC |
| Suggested primer 2: | AACGCACATACACCAGGACA |