| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148976 |
| Chromosome: | chromosome 14 |
| Location: | 2624620 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g626000 | (1 of 6) PF03547 - Membrane transport protein (Mem_trans) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTAAACGGGGTGGTAGGGTTGGGTTCAGC |
| Internal bar code: | AAGAAGCACAGGGTCTTACGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 512 |
| LEAP-Seq percent confirming: | 99.8336 |
| LEAP-Seq n confirming: | 1200 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTAGCGCACAAGAGCAGTG |
| Suggested primer 2: | TGTCAGCATCTTTCAGGTCG |