| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148980 |
| Chromosome: | chromosome 7 |
| Location: | 5729235 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g352800 | (1 of 1) PTHR35460:SF1 - TRANSLATION ELONGATION FACTOR EF-1 ALPHA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGTTCCCTGCATGCCTTCAATCGCACCCCC |
| Internal bar code: | ACCAGGCCTTGCCCGCAAATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 142 |
| LEAP-Seq percent confirming: | 81.4208 |
| LEAP-Seq n confirming: | 149 |
| LEAP-Seq n nonconfirming: | 34 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCACACACACTATCGCCC |
| Suggested primer 2: | GCACACACTCGCACAGTCTT |