| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.148986 |
| Chromosome: | chromosome 2 |
| Location: | 5683891 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g109850 | PIGC,SETH1,PIGC1,GPI2,GTR18 | (1 of 1) K03859 - phosphatidylinositol glycan, class C (PIGC, GPI2); Phosphatidylinositol N-acetylglucosaminyltransferase, subunit C | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACAAAACACCACTGACGCCCCACACCCG |
| Internal bar code: | TACAAGTCGATGCTCTACCTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 660 |
| LEAP-Seq percent confirming: | 95.8498 |
| LEAP-Seq n confirming: | 485 |
| LEAP-Seq n nonconfirming: | 21 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTGTCAGATGTATGCGCC |
| Suggested primer 2: | TGCCTTGCTTATTGCATTTG |