| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.148994 |
| Chromosome: | chromosome 6 |
| Location: | 53165 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g249150 | MAPKK1 | Mitogen-Activated Protein Kinase Kinase 1; (1 of 1) PTHR24361//PTHR24361:SF408 - MITOGEN-ACTIVATED KINASE KINASE KINASE // SERINE/THREONINE-PROTEIN KINASE STE7 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGCCCGCTGAGATTGGCCCATGGGGA |
| Internal bar code: | AGACGTCAGCAGGGATGTCCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 648 |
| LEAP-Seq percent confirming: | 99.703 |
| LEAP-Seq n confirming: | 1343 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 9 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGCAAGAGCATACAGCTTC |
| Suggested primer 2: | CACACACACTCCCAATCCAG |