Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.149006 |
Chromosome: | chromosome 16 |
Location: | 565297 |
Confidence (%): | 73 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre16.g692450 | 5'UTR |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCGAGACACGGAGTCGTTGCTTCCACCT |
Internal bar code: | CAGACGTCCCGACGCCGGCGCG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1105 |
LEAP-Seq percent confirming: | 99.0116 |
LEAP-Seq n confirming: | 5209 |
LEAP-Seq n nonconfirming: | 52 |
LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CGAGTTTTGAACGGGTGTTT |
Suggested primer 2: | CTGACCTATTCACCGCACCT |