Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.149018 |
Chromosome: | chromosome_13 |
Location: | 1497847 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre13.g572700 | FLA15,FAP66,IFT144 | Intraflagellar transport protein 144 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | GCAATCAACCTTTCGCTTCCTTCCCCGCGG |
Internal bar code: | GCGCAGCCAACGTGAATGGATG |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 1131 |
LEAP-Seq percent confirming: | 99.7728 |
LEAP-Seq n confirming: | 4830 |
LEAP-Seq n nonconfirming: | 11 |
LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTCTGACCGTCAACCCTTGT |
Suggested primer 2: | ACCTGAAGGCTTTTTGGGTT |