| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149031 |
| Chromosome: | chromosome 12 |
| Location: | 2617189 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g504950 | UO,UOX1,UOX | Urate oxidase II; (1 of 1) K00365 - urate oxidase (uaZ) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAGGTGGTGGCGTCACGGCAGGCGGCA |
| Internal bar code: | GCGCCCGGCTTGCGCTGCGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 432 |
| LEAP-Seq percent confirming: | 99.6746 |
| LEAP-Seq n confirming: | 919 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATCCTCCTTCAACAACGA |
| Suggested primer 2: | CTCATCTGATGGCAGCTTCA |