Insertion junction: LMJ.RY0402.149043_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.149043
Chromosome:chromosome 16
Location:3647996
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre16.g688450 CFAP298,DAB2,C21ORF59,FBB18 (1 of 1) PF11069 - Protein of unknown function (DUF2870) (DUF2870); Flagellar basal body proteome 18 / Dynein assembly factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTGGTGTACGCGTCATCCTCGTCCTCTGC
Internal bar code:AGATCTCTAAAAGACAATCATA

Confirmation - LEAP-Seq

LEAP-Seq distance:757
LEAP-Seq percent confirming:99.6505
LEAP-Seq n confirming:3421
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:CCATATCCATATGCACGCTG
Suggested primer 2:CAAGTCTGATTCAGCAGGCA