| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149049 |
| Chromosome: | chromosome 9 |
| Location: | 7401527 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g413200 | STPK22,STK22 | Serine/threonine protein kinase; (1 of 18) K08824 - cyclin-dependent kinase-like [EC:2.7.11.22] (CDKL) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACCAACGCAATGACGAACCGTTCGTACC |
| Internal bar code: | GACTACAGACACTGAGCGCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 469 |
| LEAP-Seq percent confirming: | 99.5029 |
| LEAP-Seq n confirming: | 1201 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGATGGGGCTGACGTTATG |
| Suggested primer 2: | GTAAAGGTGCAGCTTCTGGC |