| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149064 |
| Chromosome: | chromosome 7 |
| Location: | 2531517 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g329750 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTCATTGTCCTGCCTCATGACAATCCTTC |
| Internal bar code: | GCGCGCAAGCTGTCAACGGAAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1004 |
| LEAP-Seq percent confirming: | 99.3889 |
| LEAP-Seq n confirming: | 3578 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGAGAGAAGAGCGAGCGAG |
| Suggested primer 2: | AAGGTACCGGCACTGAACAC |