| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149084 |
| Chromosome: | chromosome 13 |
| Location: | 1644360 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g573800 | (1 of 2) 2.4.2.9//2.7.1.48 - Uracil phosphoribosyltransferase / UMP pyrophosphorylase // Uridine kinase / Uridine monophosphokinase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTTGATTCTCACAAGCTATATCCCACCCA |
| Internal bar code: | TCTGCCTTCAGTATTTTCCAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGATTGACTCCGTTCCCTT |
| Suggested primer 2: | GTGCTCCGTGTACTGCTTCA |