| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149093 |
| Chromosome: | chromosome 3 |
| Location: | 9137335 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g211969 | gene_edge/mRNA_edge/5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAAAATGCAGCCAGGATGCAAAGTGGCTAT |
| Internal bar code: | AGAAACCGCCGTAACCTTTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1167 |
| LEAP-Seq percent confirming: | 99.6381 |
| LEAP-Seq n confirming: | 1927 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGAGCTCCAAGGATTGAGG |
| Suggested primer 2: | TTTCAGACCAGATGACTGCG |