| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149106 |
| Chromosome: | chromosome 13 |
| Location: | 4737914 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g604850 | (1 of 1) IPR026126 - BRISC and BRCA1-A complex member 1 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGCGTGGGAAGGACGGGAGGACGTCGCA |
| Internal bar code: | GGTATAATCCGGCCGGGTAGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 140 |
| LEAP-Seq percent confirming: | 99.8016 |
| LEAP-Seq n confirming: | 503 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGAGAGGGTGTGTGTGTGTG |
| Suggested primer 2: | ACGCACACATACACCAGCAT |