Insertion junction: LMJ.RY0402.149111_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.149111
Chromosome:chromosome 10
Location:974319
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre10.g424775 CTP2,PCH1,PAA1 Copper transporting ATPase and Cu chaperone for CTP1; (1 of 1) PTHR24093:SF124 - COPPER-TRANSPORTING ATPASE PAA1, CHLOROPLASTIC CDS|outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCTGGTTGATCTTGGCAAGTGTGCGCTT
Internal bar code:ACGTCGTAGGTACCGGTCCGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:458
LEAP-Seq percent confirming:99.1304
LEAP-Seq n confirming:342
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:ATCGGATCGGTCAGAACTTG
Suggested primer 2:GCAGTCGTGTCGCTCAAATA