Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.149124 |
Chromosome: | chromosome_12 |
Location: | 3373120 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre12.g497200 | FAP216 | Flagellar Associated Protein | sense | 5'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTGAGGGCACAAGACAACCGCCCACAACAA |
Internal bar code: | CATGGGAGGATGTCACTTAGAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 953 |
LEAP-Seq percent confirming: | 99.8682 |
LEAP-Seq n confirming: | 1516 |
LEAP-Seq n nonconfirming: | 2 |
LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GCGGAGGATTGGAAGTCATA |
Suggested primer 2: | GTATGGGACCAAGGCGTAGA |