| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149124 |
| Chromosome: | chromosome 16 |
| Location: | 1338729 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g651801 | (1 of 7) IPR000104//IPR020683 - Antifreeze protein, type I // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCGAAGCCATCGCCGAGTTTGCCCGCC |
| Internal bar code: | ATGCGGTGCCCTGGGGGACGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 280 |
| LEAP-Seq percent confirming: | 99.784 |
| LEAP-Seq n confirming: | 462 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCAAAGCTGGACGACGTA |
| Suggested primer 2: | CTTCTGGTCTGCCTACTCCG |