| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149143 |
| Chromosome: | chromosome 7 |
| Location: | 6386623 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g357750 | (1 of 3) PTHR10383 - SERINE INCORPORATOR | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTTCGTGACGTGGAACCTCATCATTGGT |
| Internal bar code: | AAGTCGCATACATCTATCGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 897 |
| LEAP-Seq percent confirming: | 99.649 |
| LEAP-Seq n confirming: | 9936 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATACCTGGGGGTAGAGGTGG |
| Suggested primer 2: | AACTTCGTGTACGAGGTGGG |