| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149151 |
| Chromosome: | chromosome 8 |
| Location: | 4610257 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre08.g383350 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCACAAGGCTGCAACTAGCGGCTGAAAGG |
| Internal bar code: | CTATGGGATCGTCGGAAACACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 974 |
| LEAP-Seq percent confirming: | 99.0712 |
| LEAP-Seq n confirming: | 13867 |
| LEAP-Seq n nonconfirming: | 130 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGACACGGCACACAGGAT |
| Suggested primer 2: | GGACCGATGCTTGATCTTGT |