| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.149169 |
| Chromosome: | chromosome 5 |
| Location: | 3229694 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g239978 | (1 of 1) PTHR36116//PTHR36116:SF1 - FAMILY NOT NAMED // UPF0060 MEMBRANE PROTEIN YNFA | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCGCTCGTTCGTACACAGGCAAAGCTAG |
| Internal bar code: | CCACTACTCCGGAGCGATTTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1018 |
| LEAP-Seq percent confirming: | 99.2749 |
| LEAP-Seq n confirming: | 3149 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACCTGGTGGCTGGTAGATG |
| Suggested primer 2: | GGTCAGGGTCATCTACGCAT |