| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.149183 |
| Chromosome: | chromosome_14 |
| Location: | 1869056 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| None |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GGGCGTACGACTCGTTCGACCAGCCGGGCC |
| Internal bar code: | GCTGCGACTCGTTCCGGCATGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 957 |
| LEAP-Seq percent confirming: | 99.4511 |
| LEAP-Seq n confirming: | 906 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GACGAAATCGAGCACAGTGA |
| Suggested primer 2: | GCCTTGGAGCAGCAGTAATC |