| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.149185 |
| Chromosome: | chromosome 12 |
| Location: | 3136514 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g499650 | (1 of 2) PF06127 - Protein of unknown function (DUF962) (DUF962) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCACCCGCACGCCCCGGCCACCCAGCAA |
| Internal bar code: | CAGTTTTCGTAGCTAGCACTCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 567 |
| LEAP-Seq percent confirming: | 98.8701 |
| LEAP-Seq n confirming: | 350 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGTCAGGTGGAAAAGCAT |
| Suggested primer 2: | ATCACCCAGCGTCTGGATAC |