| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.149202 |
| Chromosome: | chromosome 2 |
| Location: | 4128981 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g097900 | AST3 | (1 of 1) K00811 - aspartate aminotransferase, chloroplastic (ASP5); Aspartate aminotransferase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCTGATGCATTTGTCCAGGCGTGAGGCG |
| Internal bar code: | CGTGCACGCGGTTCATAATCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 789 |
| LEAP-Seq percent confirming: | 99.4166 |
| LEAP-Seq n confirming: | 2556 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AACGCTTTATGTGGACCAGG |
| Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |