Insertion junction: LMJ.RY0402.149244_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.149244
Chromosome:chromosome 17
Location:4128041
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre17.g729650 MPA15 Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR35769:SF2 - CALCINEURIN-LIKE METALLO-PHOSPHOESTERASE SUPERFAMILY PROTEIN CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCGAGGAGGTGGGTCACGTGGGTGGGTGG
Internal bar code:GCGGCGTGTGTCATCCGGGCGG

Confirmation - LEAP-Seq

LEAP-Seq distance:756
LEAP-Seq percent confirming:99.3111
LEAP-Seq n confirming:12686
LEAP-Seq n nonconfirming:88
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:TTGGGCCCTAGACAGTAACG
Suggested primer 2:GCGCTGAGAAAAGGTACCAG